Fish endemic to only a single lake! (19 species added)
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The following species were added on 23 July 2023:
- Stone sculpin (Paracottus knerii)
- Lake Biwa gudgeon (Sarcocheilichthys biwaensis)
- Lake sturgeon (Acipenser fulvescens)
- Japanese mystery snail (Cipangopaludina japonica)
- Yodo dwarf gudgeon (Biwia yodoensis)
- Moore's lamprologus (Variabilichromis moorii)
- Aobara blue belly goby (Rhinogobius sp. 'BB')
- Western hotoke loach (Lefua torrentis)
- Biwa-higai gudgeon (Sarcocheilichthys variegatus microoculus)
- Lake Biwa hydrozoan (Pachycordyle kubotai)
- Siberian taimen (Hucho taimen)
- Willow gudgeon (Gnathopogon caerulescens)
- Biwa trout (Oncorhynchus rhodurus)
- Baikal sturgeon (Acipenser baerii 'baicalensis')
- Oumi spined loach (Cobitis minamorii oumiensis)
- Threadfin cichlid (Petrochromis trewavasae)
- Giant Lake Biwa catfish (Silurus biwaensis)
- Isaza goby (Gymnogobius isaza)
- Big red sculpin (Procottus major)
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